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CURRICULUM VITAE |
NAME
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Chen, Di |
POSITION TITLE
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Professor of Orthopaedics |
EDUCATION/TRAINING
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| Tianjin Medical University, China M.D. 1982 Medicine |
| University of Louisville, Kentucky Ph.D. 1992 Pharmacology/Toxicolog |
Univ of Texas Health Sci Ctr at San Antonio Post-doc 1992-1995 Endocrinology
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| POSITIONS |
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| 1995-1998 |
Research Scientist, OsteoScreen Inc., San Antonio, Texas |
| 1998-2003 |
Senior Scientist, OsteoScreen Inc., San Antonio, Texas |
| 1998-2003 |
Adjunct Assistant Professors |
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Department of Medicine, Division of Endocrinology |
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Department of Cellular and Structural Biology |
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University of Texas Health Science Center at San Antonio, San Antonio, Texas |
| 2003-2007 |
Assistant Professor, Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine, Rochester, New York |
2007-present
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Professor with tenure, Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine, Rochester, New York |
PROFESSIONAL MEMBERSHIPS |
| Member, American Society for Bone and Mineral Research (ASBMR) (1992-present) |
| Member, Education Committee, ASBMR (2007-2010) |
| Member, International Bone and Mineral Society (IBMS) (1998-present) |
| Member, Orthopaedic Research Society (ORS) (2003-present) |
| Co-Chair, Bone Topic Committee, ORS (2007-2009) |
| Member, New York Academy of Science (NYAS) (2005-present) |
| Member of board of director, International Chinese Hard Tissue Society (ICHTS) (2000-2006) |
| Vice President, ICHTS (2001-2005) |
| President, ICHTS (2005-2007) |
HONORS AND AWARDS |
| 1. Fellowship (1986-1987), University of Louisville |
2. Travel award (2002), Ninth Workshop on Cell Biology of Bone and Cartilage in Health and
Disease, Davos, Switzerland. |
| 3. Outstanding Paper Award (2002), International Osteoporosis Conference, Shanghai, China. |
4. Travel award (2003), Biennial scientific meeting of the International Bone and Mineral Society
(IBMS), Osaka, Japan |
5. Novartis Young Investigator Award (2005), Second Joint Meeting of the European Calcified
Tissue, Society (ECTS) and International Bone and Mineral Society (IBMS), June 25-29,
Geneva, Switzerland |
| 6. Independent Scientist Award (2006-2011), NIH/NIAMS |
| 7. Visiting Fellow, ANZAC Research Institute, University of Sydney, Oct-Nov, 2006; April, 2007 |
| PUBLICATIONS |
| Original Articles: |
1. Zhou YY, Tang ZG, Jing LP, He JH, Chen D, Hou DY, and Chu L (1987) Infusing drug concentration- and
speed-response curves. Acta Pharma Sinic 8:385-389.
2. Yamamoto T, Pierce Jr. WM, Hurst HE, Chen D, and Waddell WJ (1988) Inhibition of the metabolism of
urethane by ethanol. Drug Metab Dispos 16:355-358.
3. Yamamoto T, Pierce Jr. WM, Hurst HE, Chen D, and Waddell WJ (1990) Ethyl carbamate metabolism: in
vivo inhibitors and in vitro enzymatic systems. Drug Metab Dispos 18:276-280.
4. Qi X, Chen D, Nottin R, Mace L, Herve P, and Weiss M (1993) Evidence of L-arginine/nitric oxide
pathway in endothelium and smooth muscle of human internal mammary artery. Biochem Biophys Res
Co 195:90-96.
5. Pierce Jr. WM, Sharir M, Waite KJ, Chen D, and Kaysinger KK (1993) Topically active ocular carbonic
anhydrase inhibitors: Novel biscarbonylamidothiadiazole sulfonamides as ocular hypotensive agents. P
Soc Exp Biol Med 203:360-365.
6. Chen D, Feng JQ, Feng M, Harris MA, Mundy GR, and Harris SE (1993) Cloning and sequence of bone
morphogenetic protein 4 cDNA from fetal rat calvarial cells. Biochim Biophys Acta 1174:289-292.
7. Sharir M, Pierce Jr. WM, Chen D, and Zimmerman TJ (1994) Pharmacokinetics, acid-base balance and
intraocular pressure effects of ethyloylazolamide - A novel topically active carbonic anhydrase inhibitor.
Exp Eye Res 58:107-116.
8. Feng JQ, Chen D, Esparza J, Harris MA, Mundy GR, and Harris SE (1995) Deer antler tissue contains
two types of bone morphogenetic protein 4 mRNA transcripts. BBA-Gene Struct Expr 1263:163-168.
9. Feng JQ, Chen D, Cooney A, Tsai MJ, Harris MA, Tsai SY, Feng M, Mundy GR, and Harris SE (1995)
The mouse bone morphogenetic protein 4-gene: Analysis of promoter utilization in fetal rat calvarial
osteoblasts and regulation by COUP-TFI orphan receptor. J Biol Chem 270:28364-28374.
10. Chen D, Feng JQ, Feng M, Harris MA, Mahy P, Mundy GR, and Harris SE (1995) Sequence and
expression of bone morphogenetic protein 3 mRNA in prolonged cultures of fetal rat calvarial osteoblasts
and in rat prostate adenocarcinoma PA III cells. DNA Cell Biol 14:235-239.
11. Chen D, Chen H, Feng JQ, Windle JJ, Koop BA, Harris MA, Bonewald LF, Boyce BF, Wozney JM,
Mundy GR, and Harris SE (1995) Osteoblastic cell lines derived from a transgenic mouse containing the
osteocalcin promoter driving SV40 T-antigen. Mol Cell Differ 3:193-212.
12. Garcia C, Qiao M, Chen D, Kirchen M, Gallwitz W, Mundy GR, and Bonewald LF (1996) Effects of
synthetic peptido-leukotrienes on bone resorption in vitro. J Bone Miner Res 11:521-529.
13. Feng JQ, Chen D, Ghosh-Choudhury N, Esparza J, Mundy GR, and Harris SE (1997) Bone
morphogenetic protein 2 transcripts in rapidly developing deer antler tissue contain an extended 5'
non-coding region arising from a distal promoter. BBA-Gene Struct Expr 1350:47-52.
14. Chen D, Harris MA, Rossini G, Dunstan CR, Dallas SL, Feng JQ, Mundy GR, and Harris SE (1997) Bone
morphogenetic protein 2 (BMP-2) enhances BMP-3, BMP-4 and bone cell differentiation marker gene
expression during the induction of mineralized bone matrix formation in cultures of fetal rat calvarial
osteoblasts. Calcif Tissue Int 60:283-290.
15. Hoang AM, Chen D, Oates TW, Jiang C, Harris SE, and Cochran DL (1997) Development and
characterization of a transformed human periodontal ligament cell line. J Periodontol 68:1054-1062.
16. Chen D, Waite LC, and Pierce Jr. WM (1998) In vitro bone resorption is dependent on physiological
concentrations of zinc. Biol Trace Elem Res 61:9-18.
17. Mbalaviele G, Nishimura R, Myoi A, Niewolna M, Reddy SV, Chen D, Feng JQ, Roodman GD, Mundy
GR, and Yoneda T (1998) Cadherin-6 mediates the heterotypic interactions between the hemopoietic
osteoclast cell lineage and stromal cells in a murine model of osteoclast differentiation. J Cell Biol
141:1467-1476.
18. Nishimura R, Kato Y, Chen D, Harris SE, Mundy GR, and Yoneda T (1998) Samd5 and DPC4 are key
molecules in mediating BMP-2-induced osteoblastic differentiation of the pluripotent mesenchymal
precursor cell line C2C12. J Biol Chem 273:1872-1879.
19. Chen D, Ji X, Harris MA, Feng JQ, Celeste AJ, Rosen V, Mundy GR, and Harris SE (1998) Differential
roles for BMP receptor type IB and IA in differentiation and specification of mesenchymal precursor cells
to osteoblast and adipocyte lineages. J Cell Biol 142:295-305.
20. Jikko A, Harris SE, Chen D, Mendrick DL, and Damsky CH (1999) Collagen integrin receptors regulate
early osteoblast differentiation induced by BMP-2. J Bone Miner Res 14:1075-1083.
21. Chen D, Waite LC, and Pierce Jr. WM (1999) In vitro effects of zinc on markers of bone formation. Biol
Trace Elem Res 68:225-234.
22. Shi X, Yang X, Chang Z, Chen D, and Cao X (1999) Smad1 interacts with homeodomain DNA binding
proteins in BMP signaling. J Biol Chem 274:13711-13717.
23. Mundy GR, Garrett R, Harris SE, Chan J, Chen D, Rossini G, Boyce B, Zhao M, and Gutierrez G (1999)
Stimulation of bone formation in vitro and in rodents by statins. Science 286:1946-1949.
24. Ji X, Chen D, Xu C, Harris SE, Mundy GR, and Yoneda T (2000) Patterns of gene expression associated
with BMP-2-induced osteoblast and adipocyte differentiation of mesenchymal progenitor cell 3T3-F442A.
J Bone Miner Metab 18:132-139.
25. Zhao M, Harris SE, Horn D, Geng Z, Nishimura R, Mundy GR, and Chen D (2002) BMP receptor
signaling is necessary for normal murine postnatal bone formation. J Cell Biol 157:1049-1060.
26. Garrett IR, Chen D, Gutierrez G, Rossini G, Zhao M, Escobedo A, Kim KB, Hu S, Crews CM, and Mundy
GR (2003) Selective inhibitors of the osteoblast proteasome stimulate bone formation in vivo and in vitro.
J Clin Invest 111:1771-1782.
27. Oyajobi BO, Franchin G, Williams PJ, Pulkrabek D, Gupta A, Munoz S, Grubbs B, Zhao M, Chen D,
Sherry BA, and Mundy GR (2003) Dual effects of macrophage inflammatory protein-1α on osteolysis and
tumor burden in the murine 5TGM1 model of myeloma bone disease. Blood 102:311-319.
28. Zhao M, Qiao M, Oyajobi B, Mundy GR, and Chen D (2003) E3 ubiquitin ligase Smurf1 mediates
core-binding factor alpha 1/Runx2 degradation and plays a specific role in osteoblast differentiation. J
Biol Chem 278:27939-27944.
29. Feng JQ, Xing L, Zhang J, Zhao M, Horn D, Chan J, Boyce BF, Harris SE, Mundy GR and Chen D (2003)
NF-kB specifically activates BMP-2 gene expression in growth plate chondrocytes in vivo and in a
chondrocyte cell line in vitro. J Biol Chem 278:29130-29135.
30. Rowe PS, Kumagai Y, Gutierrez G, Garrett IR, Blacker R, Rosen D, Cundy J, Navvab S, Chen D,
Drezner MK, Quarles LD, and Mundy GR (2004) MEPE has the properties of an osteoblastic
phosphatonin and minhibin. Bone 34:303-319.
31. Zhao M, Qiao M, Harris SE, Oyajobi BO, Mundy GR, and Chen D (2004) Smurf1 inhibits osteoblast
differentiation and bone formation in vitro and in vivo. J Biol Chem 279:12854-12859.
32. Mlbalaviele G, Sheikh S, Stains JP, Salazar VS, Cheng SL, Chen D, and Civitelli R (2005) Beta-catenin
and BMP-2 synergize to promote osteoblast differentiation and new bone formation. J Cell Biochem
94:403-418.
33. Ye L, Mishina Y, Chen D, Huang H, Dallas SL, Dallas M, Kunieda TW, Tsutsui T, Boskey A, Bonewald LF
and Feng JQ (2005) Dentin matrix protein 1 (Dmp1) deficient mice display severe defects in cartilage
formation responsible for a chondrodysplasia-like phenotype J Biol Chem 280:6197-6203.
34. Dong Y, Drissi H, Chen M, Chen D, Zuscik MJ, Schwarz EM, and O’Keefe RJ (2005) Wnt-mediated
regulation of chondrocyte maturation: Modulation by TGF-beta. J Cell Biochem 95:1057-1068.
35. Li TF, Darowish M, Zuscik MJ, Chen D, Schwarz EM, Rosier RN, Drissi H, O'keefe RJ (2005)
Smad3-deficient chondrocytes have enhanced BMP signaling and accelerated differentiation. J Bone
Miner Res 21:4-16.
36. Young N, Mikhalkevich N, Yan Y, Chen D, and Zheng WP (2005) Differential regulation of osteoblast
activity by Th cell subsets mediated by parathyroid hormone and IFN-gamma. J Immunol 175:8287-8295.
37. Shen R, Chen M, Wang Y, Kaneki H, Xing L, O’Keefe RJ and Chen D (2006) Smad6 interacts with Runx2
and mediates Smurf1-induced Runx2 degradation. J Biol Chem 281:3569-3576.
38. Kaneki H, Gao R, Chen D, Yao Z, Schwarz EM, Zhang YE, Boyce BF, and Xing L (2006) TNF promotes
Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts. J Biol Chem
281:4326-4333.
39. Shen R, Wang X, Drissi H, Liu F, O’Keefe RJ, and Chen D (2006) Cyclin D1-Cdk4 induce Runx2
ubiquitination and degradation. J Biol Chem 281:16347-16353.
40. O'Keefe RJ, Tiyapatanaputi P, Xie C, Li TF, Clark C, Zuscik MJ, Chen D, Drissi H, Schwarz E, and Zhang
X (2006) COX-2 has a critical role during incorporation of structural bone allografts. Ann NY Acad Sci
1068:532-542.
41. Li T, Chen D, Wu Q, Chen M, Sheu T, Schwarz EM, Drissi H, Zuscik M, and O’Keefe RJ (2006) TGF-beta
stimulates cyclin D1 expression through activation of beta-catenin signaling in chondrocytes. J Biol Chem
281:21296-21304.
42. Zhao M, Qiao M, Harris SE, Chen D, Oyajobi BO and Mundy GR (2006) The zinc finger transcription
factor Gli2 mediates bone morphogenetic protein 2 expression in osteoblasts in response to hedgehog
signaling. Mol Cell Biol 26:6197-6208.
43. Robertson G, Xie C, Chen D, Schwarz E, O’Keefe RJ, Guldberg R, and Xinping Zhang X (2006)
Alteration of femoral bone morphology and density in COX2-/- mice. Bone 39:767-772.
44. Chen M, Lichtler AC, Sheu T, Xie C, Zhang X, O’Keefe RJ and Chen D (2007) Generation of a transgenic
mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase.
Genesis 45:44-50.
45. Wang Q, Wei X, Zhu T, Zhang M, Shen R, Xing L, O’Keefe RJ, and Chen D (2007) BMP-2 activates
Smad6 gene transcription through bone-specific transcription factor Runx2 J Biol Chem
282:10742-10748.
46. Wang Q, Zhang M, Wang X, Yuan W, Chen D, Royer-Pokora B, and Zhu T (2007) A novel transcript of
the LMO2 gene, LMO2-c, is regulated by GATA-1 and PU.1 and encodes an antagonist of LMO2.
Leukemia 21:1015-1025.
47. Tan X, Weng T, Zhang J, Wang J, Li W, Wan H, Lan Y, Cheng X, Hou N, Liu H, Ding J, Lin F, Yang R,
Gao X, Chen D, and Yang X (2007) SMAD4 is required for maintaining normal murine postnatal bone
homeostasis J Cell Sci 120:2162-70.
48. Zhu M, Chen M, Lichtler AC, O’Keefe RJ, and Chen D (2007) Tamoxifen-inducible Cre-recombination in
articular chondrocytes of adult Col2a1-CreERT2 transgenic mice. Osteoarthr Cartilage (in press)
49. Usui M, Xing L, Drissi H, Zuscik M, O’Keefe RJ, Chen D, and Boyce BF (2007) Murine and chicken
chondrocytes regulate osteoclastogenesis by producing RANKL in response to BMP-2. J Bone Miner
Res (in press)
50. Dao D, Yang X, Chen D, Zuscik M, and O’Keefe RJ (2007) Axin1 and Axin2 are regulated by TGF-β and
mediate crosstalk between TGF-β and Wnt signaling pathways. Ann NY Acad Sci (in press)
51. Zhu M, Chen M, Zuscik M, Wu Q, Wang Y, Rosier RN, O’Keefe RJ and Chen D (2007) Inhibition of
β-catenin signaling in articular chondrocytes results in the development of osteoarthritis. Arth Rheum (in
press)
52. Chen M, Zhu M, Awad H, Sheu T, Boyce BF, Chen D, and O’Keefe RJ (2007) Inhibition of β-catenin
signaling causes defects in postnatal cartilage development. J Cell Sci (in press)
Reviews:
1. Qi X and Chen D (1993) Endothelium-dependent relaxation and interaction between indomethacin and
L-NG-monomethylarginine in coronary bypass grafts. J Thorac Cardiovasc Surg 106:563-564. Letter to
Editor.
2. Mundy GR, Boyce BF, Hughes D, Wright K, Bonewald LF, Dallas SL, Harris SE, Ghosh-Choudhury N,
Chen D, Dunstan CR, Izbicka E, and Yoneda T (1995) The effects of cytokines and growth factors on
osteoblastic cells. Bone 17:71-75S.
3. Chen D and Ye W (1996) Bone formation and clinical applications of bone morphogenetic proteins. Chin
J Surg 34:602-605.
4. Chen D, Ye W, and Cai F (1999) Bone morphogenetic protein receptors and signal transduction. Prog
Biochem Biophys 26:141-143.
5. Mundy GR, Chen D, Zhao M, Dallas S, Xu C, and Harris SE (2001) Growth regulatory factors and bone.
Rev Endocr Metab Disord 2:105-115.
6. Chen D, Zhao M, Harris SE, and Mi Z (2004) BMP receptor signaling and their biological functions. Front
Biosci 9:349-358.
7. Chen D, Zhao M, and Mundy GR (2004) Bone morphogenetic proteins. Growth Factors 22:233-241.
8. Li T, O’Keefe RJ and Chen D (2005) TGF-beta signaling in chondrocytes. Front Biosci 10:681-688.
9. Cao X and Chen D (2005) The BMP signaling and in vivo bone formation. Gene 357:1-8.
10. Boyce BF, Xing L, and Chen D (2005) Osteoprotegerin, the bone protector, is a surprising target for
β-catenin signaling. Cell Metab 2:344-345. Preview.
11. Wang Q, Zhu T, Zhang M, and Chen D (2006) The role of E3 ubiquitin ligase Smurfs in BMP and TGF-β
signaling in bone cells. Prog Biochem Biophys 33:423-430.
Book Chapters:
1. Chen D, Zhao M, Oyajobi B, and Mundy GR (2000) Update in Bone Cell Biology. In: Osteoporosis and
Bone Biology. Compston J and Ralston S (eds), International Medical Press, United Kingdom, p29-41.
2. Chen D and Geng Z (2001) Molecular mechanism of BMP signaling pathway and bone formation. In:
Osteoporosis, Basic and Clinical Aspects. Guo S, Luo X, and Qiu G (eds), Tianjin Science and
Technology Press, p154-163.
3. Mundy GR, Oyajobi B, Traianedes K, Dallas S, and Chen D (2001) Cytokines and bone remodeling. In:
Osteoporosis (2nd edition), Marcus B, Kelsey J, and Feldman D (eds) Academic Press, San Diego,
p373-403.
4. Mundy GR, Gutierrez G, Gallwitz, Feng J, Chen D, Garrett R, and Harris S (2001) Antler-derived bone
growth factors and their potential for use in osteoporosis. In: Antler Science and Product Technology. Sim
JS, Sunwoo HH, Hudson RJ, and Jeon BT (eds) ASPTRC press, Canada, p171-187.
5. Zhao M and Chen D (2002) Expression of rhBMP-2 in Escherichia coli and Its Activity in Inducing Bone
Formation. In: Advances in Skeletal Reconstruction Using Bone Morphogenetic Proteins. Lindholm TS
(ed) World Scientific Publishing, New Jersey, London, Singapore, p1-18.
6. Chen D, Zhao M, and Mundy GR (2003) Bone morphogenetic proteins. In: Encyclopedia of Hormones,
Growth Factors and Cytokines. Norman A and Henry H (eds) Academic Press, Academic Press Inc., San
Diego p205-209.
7. Oyajobi B, Chen D and Mundy GR (2003) Bone remodeling. In: Primer on the Metabolic Bone Diseases
and Disorders of Mineral Metabolism (5th edition). Favus MJ (ed), Lippincott William & Wilkins,
Philadelphia p46-58.
8. Chen D, Yan Y, Chen M, Shen H, and Deng HW (2005) Recent advances in bone biology research. In:
Current Topics in Bone Biology. Deng HW and Liu YZ (eds), World Scientific Publishing Co., Singapore,
p497-511.
9. Chen D, Chen M, Yan Y, Wang Y, and Zhu T (2005) Regulation of bone remodeling. In: Current Topics in
Bone Biology. Deng HW and Liu YZ (eds), World Scientific Publishing Co., Singapore, p279-298.
10. Li T, O’Keefe RJ, and Chen D (2005) TGF-beta in chondrocyte biology and cartilage pathology. In:
Current Topics in Bone Biology. Deng HW and Liu YZ (eds), World Scientific Publishing Co., Singapore,
p299-311.
11. Chen D and Schwarz EM (2006) Signal transduction in rheumatic diseases. In: Rheumatoid Arthritis:
New frontier in pathogenesis and treatment. Firestein GS, Panayi GS, and Wollheim FA (eds), Oxford
University Press, Oxford, UK, p541-550. |
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