CURRICULUM VITAE
NAME Gian-Paolo Dotto
EMAIL ADDRESS gdotto@partners.org / gian-paolo.dotto@unil.ch
PLACE OF BIRTH Turin, ITALY
EDUCATION
1979 M.D. Turin Medical School, Turin, Italy
1983 Ph.D. The Rockefeller University, New York NY
POSTDOCTORAL TRAINING
1983 Research Associate Fellow; with Dr. Norton D. Zinder,
The Rockefeller University, New York NY
1984 Postdoctoral Fellow; with Dr. Robert A. Weinberg, Department
of Biology, Cancer Center and Whitehead Institute, Cambridge, MA
ACADEMIC APPOINTMENTS
1987 Assistant Professor, Yale University School of Medicine, CT
1992 Associate Professor, Yale University School of Medicine, CT
1992 Associate Professor, Harvard Medical School , Boston, MA
2000-2004 Professor, Harvard Medical School , Boston, MA
2002- Professor, Institute of Biochemistry, University of Lausanne, CH
HOSPITAL APPOINTMENT
1992 Associate Biologist (Dermatology), Massachusetts General Hospital, Boston MA
1999- Member, DANA-FARBER/HARVARD CANCER CENTER
2000- Biologist (Dermatology), Massachusetts General Hospital, Boston MA
OTHER PROFESSIONAL POSITIONS AND MAJOR VISITING APPOINTMENTS
1988 Lecturer; Molecular Biophysics & Biochemistry, Yale University School of Medicine
1988 Member; the Yale Comprehensive Cancer Center
1989-91 Visiting Professor, Turin University, Italy
1992-94 Visiting Professor, Padua University, Italy
1993 Member, Biological & Biomedical Sciences Program, Harvard Medical School
1997 Visiting Professor, Turin University, Italy
MAJOR COMMITTEE ASSIGNMENTS AND REVIEWING PANELS
1989 Reviewer, Damon Runyon Fellowship Program
1990 Reviewer, Soros Foundation
1991 Special Reviewer; National Institutes of Health-Cell Biology and Physiology Study Section II
1992 Consultant, Panel on Papilloma virus cell interactions, National Cancer Institute
1994- Member, Subcommittee on the Review of Research Proposals (SRRP) and The MGH Committee on Research
1995 Special Reviewer, National Institute of Health CCRP-C Study Section
1995- Reviewer, Human Science Frontier
1995- Executive Committee, Cutaneous Biology Research Center
1996 Reviewer, The Israel Science Foundation
1996- Reviewer, United States-Israel Binational Science Foundation
1996 Reviewer, National Institute of Health, Internal Review of the
Laboratory of Cellular Development and Oncology
1996 Reviewer, National Alopecia Areata Foundation
1996 Reviewer, NIH Oral Biology & Medicine Study Section-1 (ZRG4-OBM-1)
1996 Reviewer, Department of Veterans Affairs, VA Palo Alto Health Care
SYSTEM-LIVERMORE DIVISION
1998 Reviewer, NIH, NIAMS, Special Emphasis Panel Program Project
CommitteeReview Section
1998 Reviewer, NIEHS, Board of Scientific Councelors
1998- Reviewer, Italian Ministry of University and Research International
Grant Review Program
1998 Reviewer, NIAMS, Skin Disease Research Center Review Program.
1998 Member, Harvard MIT HST Graduate Program Committee
1999 Reviewer, NIH, NIAMS, Special Program Project Review Committee
2000 Member, Scientific Advisory Committee, Melanoma Research
FOUNDATION
2000 Reviewer, Department of Veterans Affair, Office of External Reviews,
VA Palo Alto Health Care System
2000 Reviewer, Extramural Programs NIH, NIAMS, K01/K08 program
2000 Reviewer, Extramural Programs NIH, NIAMS, R03 program
2000 Reviewer, Wellcome Trust Foundation, UK
2000 Reviewer, Extramural Programs NIH, ZRG1 GMA-1 Study Section
2001 Member, NIH, NIAMS, ZAR1 RJB-C Special Emphasis Panel
2001 Reviewer, Swiss National Science Foundation
2001 Member, NIH, NIAMS, ZAR1 JRL-B Special Emphasis Panel
2001 Reviewer, 8th Korea Science Award from the Korean Academy of Science and Technology
2002 Reviewer, Natural Sciences and Engineering Research Council of Canada (NSERC)
2002 Member, NIH, NIAMS, ZAR1 JRL-B Special Emphasis Panel
2002 Member, NIH GMA-1 study section
2003 Member, NIH GMA-1 study section
2003 Reviewer, Swiss National Science Foundation
2004 Reviewer, Associazione Italiana Ricerca sul Cancro, AIRC
2004 - 2006 Permanent member, NIH ACTS study section
2005 Reviewer, NIH ZRG1 MOSS D12 study section
2005 Reviewer, Swiss National Science Foundation
2005 Member, Cancer Research UK Quinquennial Review Panel, Edingburgh
PROFESSIONAL SOCIETIES
Memberships, Offices, and Committee Assignments:
1990 - Member, American Association for the Advancement of Science
1992 - Member, American Association for Cancer Research
1996 - Member, Society of Investigative Dermatology
Editorial Boards:
1999-2004 Associate Editor, Cancer Research
1997 -2002 Associate Editor, Journal of Investigative Dermatology
1998- Editorial Board Member, Cell Growth and Differentiation
1998- Editorial Board Member, Laboratory Investigation
Reviewer: Am J Pathology, Cancer Research, Cell, Cell Growth and
Differentiation, Current Biology, Development, Developmental
Biology, Developmental Cell, Developmental Dynamics, Differentiation, EMBO Journal, EMBO reports, Exp. Cell Res., Genes and Development, Genes to Cells, J. Biol Chem, J. Cell Biol., J. Cell Physiol., J. Cell Sci., Lab. Investigation, J. Invest. Dermatol., Mol. Cell Biol., Mol. Biol. Cell, Mol. Carcinogenesis, Nature Medicine, Oncogene, Proc. Natl Acad. Sci. USA, Science
AWARDS AND HONORS
1982 Grant-in-Aid of Research of the Sigma Xi, The Scientific Research Society, New Haven, CT
1984 Fellow; The Jane Coffin Childs Memorial Fund, New Haven CT
1987 Hull Cancer Research Award
1988 Swebilius Cancer Research Award
2001 Honorary Masters’ degree, Harvard University
2005/2007 Vice Chair/Chair Gordon Conference on Epithelial Differentiation
REPORT OF RESEARCH
Dr. Dotto's major research contributions over the last 20 years have been in the field of keratinocyte stem cell renewal, differentiation and transformation. A key concept that stems from his work is that the biological function of individual cell regulatory pathways can only be understood within the specific context in which they operate. This principle is well illustrated by the frequently unexpected or even opposite role of individual signaling pathways in growth/differentiation control of keratinocytes versus other cell types. He established this to be the case for tyrosine kinases of the Src family, small Rho GTPases, the calcium-dependent Calcineurin phosphatase, and the cell cycle inhibitor p21WAF1/Cip1, which he discovered to play a direct role in control of transcription, independent of the cell cycle. In the context of this work, he has established unexpectedly close interactions with developmental signaling pathways, specifically Notch1 signaling, in control of epidermal stem cell potential, differentiation and tumor suppression. This has opened new perspectives on the complex signaling network that presides epithelial stem cell renewal and tumorigenesis.
BIBLIOGRAPHY
Original Reports:
1. Dotto GP, Enea V, Zinder ND. Functional analysis of bacteriophage fl intergenic gion. Virology 1981; 114:463-73.
2. Dotto GP, Enea V, Zinder ND. Gene II of phage fl: its functions and its products. Proc Natl Acad Sci USA 1981; 78:5421-24.
3. Dotto GP, Horiuchi K. Replication of a plasmid containing two origins of bacteriophage fl. J Mol Biol 1981; 153:169- 76.
4. Dotto GP, Horiuchi K, Jakes KS, Zinder ND. Replication origin of bacteriophage fl: two signals required for its function. J Mol Biol 1982; 162:335-43.
5. Dotto GP, Horiuchi K, Zinder ND. Initiation and termination of phage fl plus strand synthesis. Proc Natl Acad Sci USA 1982; 79:7122-26.
6. Dotto GP, Horiuchi K, Zinder ND. Signals for the initiation and termination of synthesis of the viral strand of bacteriophageophage fl. Cold Spring Harbor Symp Quant Biol 1983; XLVII,717-22.
7. Dotto GP, Zinder ND. The morphogenetic signal of Bacteriophage fl. Virology 1983; 130:252-56.
8. Dotto GP. The origin of DNA replication of bacteriophage fl. Ph.D. Thesis, The Rockefeller University Press. NY. 1983.
9. Dotto GP, Horiuchi K, Zinder ND. The functional origin of Bacteriophage fl DNA replication: its signals and domains. J Mol Biol 1984; 172:507-21.
10. Dotto GP, Zinder ND. Increased intracellular concentration of an initiator protein markedly reduces the minimal sequence required for initiation of DNA synthesis. Proc Natl Acad Sci USA 1984; 81:1336-40.
11. Dotto GP, Zinder ND. Reduction of the minimal sequence for initiation of DNA synthesis by qualitative or quantitative changes of an initiator protein. Nature 1984; 311:279-80.
12. Dotto GP, Parada LF, Weinberg RA. Specific growth response of ras-transformed embryo fibroblasts to tumor promoters. Nature 1985; 318:472-75.
13. Dotto GP, Gilman MZ, Maruyama M, Weinberg RA. c-myc and c-fos expression in differentiating mouse primary keratinocytes. EMBO J 1986; 5:2853-57.
14. Dotto GP, O'Connell J, Patskan G, Conti C, Ariza A, Slaga T. Malignant progression of papilloma-derived keratinocytes: Differential effects of the ras, neu, and p53 oncogenes. Molecular Carcinogenesis 1988; 1:171-79.
15. Dotto GP, Weinberg RA, Ariza A. Malignant transformation of mouse primary keratinocytes by HaSV and its modulation by surrounding normal cells. Proc Natl Acad Sci USA 1988; 85:6389-93.
16. Seremetis S, Inghirani G, Ferrero D, Newcomb EW, Knowles DM, Dotto GP, Dalla-Favera R. Transformation and plasmacytoid differentiation of EBV-infected human B lymphoblasts by ras oncogenes. Science 1989; 243:660-63.
17. Dotto GP, El-Fouly M, Nelson C, Trosko JE. Similar and synergistic inhibition of gap-junctional communication by ras transformation and tumor promoter treatment of mouse primary keratinocytes. Oncogene 1989; 4:637-41.
18. Dotto GP, Moellmann G, Ghosh S, Edwards M, Halaban R. Transformation of murine melanocytes by bFGF-cDNA and selected oncogenes and its suppression in a reconstituted cutaneous environment. J Cell Biol 1989; 109:3115-28.
19. Filvaroff E, Stern DF, Dotto GP. Tyrosine phosphorylation is an early and specific event involved in primary keratinocyte differentiation. Mol Cell Biol 1990; 10:1164-73.
20. Ramon y Cajal S, Shuster S, Halaban R, Filvaroff E, Dotto GP. Induction of different morphological features of malignant melanoma and pigmented lesions after transformation of murine melanocytes with bFGF-cDNA and H-Ras, myc, neu, and E1a oncogenes. J Am Path 1991; 138:349-60.
21. Missero C, Filvaroff E, Dotto GP. Induction of TGF-?1 resistance by the E1a oncogene requires binding to a specific set of cellular proteins. Proc Natl Acad Sci USA 1991; 88:3489-93.
22. Brissette JL, Kumar NM, Gilula NB, Dotto GP. The tumor promoter TPA and the ras oncogene modulate expression and phosphorylation of GAP junction proteins. Mol Cell Biol 1991; 11:5364-71.
23. Missero C, Ramon y Cajal S, Dotto GP. Escape from TGF-? control and oncogene cooperation in skin tumor development. Proc Natl Acad Sci USA 1991; 88:9613-17.
24. Leptak C, Ramon y Cajal S, Kulke R, Horwitz BH, Riese DJ II, Dotto GP, DiMaio D. Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16. J Virol 1991; 65:7078-83.
25. Florin-Christensen M, Missero C, Dotto GP, Florin-Christensen J. The E1a gene prevents inhibition of keratinocyte proliferation by dexamethasone. Exp Cell Res 1992; 203:285-88.
26. Filvaroff E, Calautti E, McCormick F, Dotto GP. Specific changes of ras-GAP and a gap-associated p62 protein during calcium-induced keratinocyte differentiation. Mol Cell Biol 1992; 12:5319-28.
27. Brissette JL, Missero C, Yuspa SH, Dotto GP. Different levels of v-Ha-Ras-p21 expression in primary keratinocytes transformed with Harvey sarcoma virus correlate with benign versus malignant behavior. Molecular Carcinogenesis 1992; 7:21-25.
28. Florin-Christensen M., Missero C., Florin-Christensen J., Tranque P., Ramon y Cajal S, Dotto GP. Counteracting effects of the E1a gene on cAMP growth inhibition. Exp Cell Res 1993; 207:57-61.
29. Missero C, Serra C , Stenn K & Dotto GP. Skin-specific expression of a truncated E1a oncoprotein binding to p105-Rb leads to abnormal hair follicle maturation without increased epidermal proliferation. J Cell Biol 1993; 121:1109-20.
30. Esherick JS, DiCunto F, Flanders KC, Missero C, Dotto GP. Transforming growth factor ?1 induction is associated with transforming growth factors ?2 and ?3 down-modulation in 12-0-tetradecanoylphorbol-13-acetate-induced skin hyperplasia. Cancer Res 1993; 53:1-6.
31. Brissette J, Kumar NM, Gilula NB, Hall JE and Dotto GP. Switch in gap junction protein expression is associated with selective changes in junctional permeability during keratinocyte differentiation. Proc Natl Acad Sci USA 1994; 91:6453-57.
32. Filvaroff E, Calautti E, Reiss M & Dotto GP. Functional evidence for an extracellular calcium receptor mechanism triggering tyrosine kinase activation associated with mouse keratinocyte differentiation. J Biol Chem 1994; 269:1-6.
33. Ramon y Cajal S, Missero C, Marchetti E, & Dotto GP. Dermal fibroblasts tumor suppression of RAS-transformed keratinocytes is associated with induction of squamous cell differentiation. Am J Path 1994; 145:846-55.
34. Park K, Bae H, Heydemann A, Roberts AB, Dotto GP, Sporn MB, Kim S-J. The E1A oncogene induces resistance to the effects of 1,25-dihydroxyvitamin D3 on inhibition of growth of mouse keratinocytes. Cancer Res 1994; 54:6087-89.
35. Missero C, Calautti E, Eckner R, Chin J, Tsai L-H, Livingston DM, Dotto GP. Involvement of the cell cycle inhibitor Cip1/WAF1 and the Ela-associated p300 protein in terminal differentiation. Proc Natl Acad Sci USA 1995; 92:5451-55.
36. Calautti E, Missero C, Stein PL, Ezzell RM, Dotto GP. fyn tyrosine kinase is involved in keratinocyte differentiation control. Genes & Development 1995; 9:2279-2291.
37. Halaban R, B?hm M, Dotto GP, Moellmann G, Cheng E, Zhang Y. Growth regulatory proteins that repress differentiation markers in melanocytes also downregulate the transcription factor microphthalmia. J Inv Derm 1996; 106:1266-1272.
38. Brissette JL, Li J, Kamimura J, Lee D, Dotto GP. The product of the mouse nude locus, Whn, regulates the balance between epithelial cell growth and differentiation. Genes & Development 1996; 10:2212-2221.
39. Missero C, DiCuntoF, Kiyokawa H, Koff A, Dotto GP. The absence of p21Cip1/WAF1 alters keratinocyte growth and differentiation and promotes ras-tumor progression. Genes & Development 1996; 10: 3065-3075.
40. Prowse DM, Bolgan L, Molnar A, Dotto, GP. Involvement of the Sp3 transcription factor in induction of p21Cip1/WAF1 in keratinocyte differentiation. J Biol Chem 1997; 272:1308-1314.
41. Kamimura J. Lee D, Baden H, Brissette J, Dotto GP. Primary mouse keratinocyte cultures contain hair follicle progenitor cells with multiple differentiation potential. J Invest Derm 1997; 109:534-540.
42. Ortel B, Brissette J, Chen N, Dotto GP, Hasan T. Differentiation-specific increase in ALA-induced protoporphyrin IX accumulation in primary mouse keratinocytes. British J. Cancer Res., 1998; 77:1744-1751.
43. Sanchez-Prieto, R., M. Quintanilla, P. Martin, M. Lleonart, A. Cano, G.P. Dotto, and S. Ramon y Cajal. In vivo antitumor effect of retrovirus-mediated gene transfer of the adenovirus E1a gene. Cancer Gene Ther., 1998; 5:215-224.
44. Di Cunto F, Topley G, Calautti E, Hsiao J, Ong L, Seth PK, Dotto GP. Inhibitory function of p21Cip1/WAF1 in differentiation of primary mouse keratinocytes independent of cell cycle control. Science, 1998; 280: 1069-1072.
45. Calautti E, Cabodi.S, Stein PL, Hatzfeld M, Kedersha N, Dotto GP. Tyrosine phosphorylation and Src-family kinases control keratinocyte cell-cell adhesion. J. Cell Biol. 1988; 141: 1449-1465.
46. Di Cunto F, Calautti E, Hsiao J, Ong L, Topley G, Turco E, Dotto GP. CRIK, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac binding protein Citron. J. Biol. Chem.1998; 273: 29706-29711.
47. Li, J., Foitzik, K., Calautti, E., Baden, H. , Doetschman, T. and G. P. Dotto. TGF?3, but not TGF-?1, protects keratinocytes against TPA-induced cell death in vitro and in vivo. J. Biol. Chem. 1999; 274: 4213-4219.
48. Foitzik, K., Paus, R., Doetschman T., and G. P. Dotto. The TGF-?2 isoform is both a required and sufficient inducer of murine hair follicle morphogenesis. Devel. Biol., 1999; 212: 278-289.
49. Topley, G. I., Okuyama, R., Gonzales, J. G., Conti, C. and G. P. Dotto. p21WAF1/Cip1 functions as a suppressor of malignant skin tumor formation, and a determinant of keratinocyte stem cell potential. Proc. Natl. Acad. Sci. USA, 1999; 96: 9089-9094.
50. Foitzik, K., Lindner, G., Mueller-Roever, S., Maurer, M., Botchkareva, N., Botchkarev, V., Handjiski, B., Metz, M., Hibino, T., Soma, T., Dotto, G. P. and Paus, R.. Control of murine hair follicle regression (catagen) by TGF-beta1 in vivo. Faseb J, 2000; 14:752-760.
51. Cabodi, S., Calautti, E., Talora, C., Kuroki, T., Stein, P.L. and G. P. Dotto. A Protein Kinase C ? / Fyn dependent pathway leading to keratinocyte growth arrest and differentiation. Mol. Cell 2000; 6:1121-1129.
52. Rangarajan, A., C. Talora, M. Nicolas, R. Okuyama, C. Mammucari, H. Oh, J. C. Aster, S. Krishna, D. Metzger, P. Chambon, L. Miele, M. Aguet, F. Radtke and G. P. Dotto. Notch signaling functions as a direct determinant of the exit of keratinocytes from the cell cycle and entry into differentiation. The EMBO Journal, 2001; 20:3427-3436.
53. Santini, M. P., C. Talora, T. Seki, L. Bolgan and G. P. Dotto. Cross talk between calcineurin, Sp1/Sp3 and NFAT in control of p21WAF1/CIP1 expression in keratinocyte differentiation. Proc. Natl. Acad. Sci. USA, 2001; 98:9575-9580.
54. Calautti E, Grossi M, Mammucari C, Aoyama Y, Pirro M, Ono Y, Li J, and G. P. Dotto. Fyn tyrosine kinase is a downstream mediator of Rho/PRK2 function in keratinocyte cell-cell adhesion. J Cell Biol., 2002; 156:137-48.
55. Seykora JT, Mei L, Dotto GP and Stein PL. Srcasm: a novel src activating and signaling molecule. J Biol Chem 2002; 277:2812-2822.
56. Oh H., Mammucari M., Nenci A., Cabodi S., Cohen S. N., and G. P. Dotto. Negative regulation of cell growth and differentiation by TSG101 through association with p21Cip1/WAF1. Proc. Natl. Acad. Sci. USA, 2002; 99:5430-5435.
57. Talora, C., Sgroi, D.C., Crum, C.P. and G. P. Dotto. Specific down-modulation of Notch1 signaling in cervical cancer cells is required for sustained HPV-E6/E7 expression and late steps of malignant transformation. Genes & Dev., 2002; 16:2252–2263.
58. Brisken C, Ayyannan A, Nguyen C, Heineman A, Reinhardt F, Jan T, Dey SK, Dotto GP, Weinberg RA. IGF-2 is a mediator of prolactin-induced morphogenesis in the breast. Dev Cell, 2002; 3:877-887.
59. Haq S, Michael A, Andreucci M, Bhattacharya K, Dotto P, Walters B, Woodgett J, Kilter H, Force T. Stabilization of beta-catenin by a Wnt-independent mechanism regulates cardiomyocyte growth. Proc Natl Acad Sci U S A. 2003; 100:4610-5.
60. Liu ZJ, Shirakawa T, Li Y, Soma A, Oka M, Dotto GP, Fairman RM, Velazquez OC, Herlyn M. Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol Cell Biol, 2003; 23:14-25.
61. Nicolas M, Wolfer A, Raj K, Kummer JA, Mill P, Van Noort M, Hui CC, Clevers H, Dotto GP, Radtke F. Notch1 functions as a tumor suppressor in mouse skin. Nat Genet., 2003; 33:416-21.
62. Itoh F, Itoh S, Goumans MJ, Valdimarsdottir G, Iso T, Dotto GP, Hamamori Y, Kedes L, Kato M, Dijke Pt P, Synergy and antagonism between Notch and BMP receptor signaling pathways in endothelial cells. EMBO J. 2004 ; 23:541-51.
63. Okuyama R, LeFort K and Dotto GP, A dynamic model of keratinocyte stem cell renewal and differentiation : role of the p21WAF1/Cip1 and Notch1 signaling pathways. J. Invest. Dermatol., 2004; 9:248-52.
64. Okuyama R, Nguyen BC, Talora C, Ogawa E, Di Vignano AT, Lioumi M, Chiorino G, Tagami H, Woo M, Dotto GP, High Commitment of Embryonic Keratinocytes to Terminal Differentiation through a Notch1-caspase 3 Regulatory Mechanism.
Dev Cell. 2004; 6:551-62.
65. Chiorino G, Acquadro F, Mello Grand M, Viscomi S, Segir R, Gasparini M, Dotto GP. Interpretation of expression-profiling results obtained from different platforms and tissue sources: examples using prostate cancer data, 2004, Eur J Cancer. 2004; 40:2592-603.
66. Mammucari, C., Tommasi di Vignano, A., Sharov, A. A., Havrda, M. C., Roop, D. R., Botchkarev, V. A., Crabtree, G. R. & Dotto, G. P., Integration of Notch1 and Calcineurin/NFAT signaling pathways in keratinocyte growth and differentiation control. Dev Cell 2005; 8:665-76.
67. Devgan V, Mammucari C, Millar SE, Brisken C & Dotto GP, p21WAF1/Cip1 is a negative transcriptional regulator of Wnt4 expression downstream of Notch1 activation. Genes & Dev. 2005; 19:1485–1495.
68. Grossi M, Hiou-Feige A, Tommasi Di Vignano A, Calautti E, Ostano P, Lee S, Chiorino G & Dotto GP, Negative control of keratinocyte differentiation by Rho/CRIK signaling coupled with up-regulation of KyoT1/2 (FHL1) expression. Proc Natl Acad Sci U S A. 2005; 102 :11313-11318 .
69. Wang J, Devgan V, Corrado M, Prabhu NS, El-Deiry WS, Riccardi.R, Pandolfi PP, Missero C, G. & Dotto GP, GITR is a p21 Cip1/WAF1 transcriptional target conferring resistance of keratinocytes to UV-induced apoptosis. J. Biol. Chem. 2005; 280 : 37725-37731.
70. Nguyen B-C, Lefort K, Mandinova A, Antonini D, Devgan V, Della Gatta G, Koster MI, Zhang Z, Wang J, Tommasi di Vignano A, Kitajewski J, Chiorino G, Roop DR, Missero C and Dotto GP, Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation. Genes & Dev. 2006; 20, 1028-42.
71. Sharov AA, Sharova TY, Mardaryev AN, Tommasi di Vignano A, Atoyan R, Weiner L, Yang S, Brissette JL, Dotto GP, Botchkarev VA. Bone morphogenetic protein signaling regulates the size of hair follicles and modulates the expression of cell cycle-associated genes. Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18166-71.
72. Ayyanan A, Civenni G, Ciarloni L, Morel C, Mueller N, Lefort K, Mandinova A, Raffoul W, Fiche M, Dotto GP, and Brisken C., Increased Wnt signaling triggers oncogenic conversion of human breast epithelial cells by a Notch-dependent mechanism. Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3799-804.
73. Ongusaha PP, Kim HG, Boswell SA, Ridley AJ, Der CJ, Dotto GP, Kim YB, Aaronson SA, Lee SW. RhoE is a pro-survival p53 target gene that inhibits ROCK I-mediated apoptosis in response to genotoxic stress. Curr Biol. 2006 Dec 19;16(24):2466-72.
74. Devgan V, Bach-Cuc N, Oh H, Dotto GP. p21Waf1/Cip1 suppresses keratinocyte differentiation independently of the cell cycle through transcriptional upregulation of the IGF-1 gene. J Biol Chem. 2006; 281, 30463-70.
75. Lefort K., Mandinova A, Ostano P., Kolev V., Calpini V., Kolfschoten I., Devgan V, Lieb J, Raffoul W, Hohl D, Neel V, Garlick J, Chiorino G, and Dotto GP. Notch1 is a p53 target gene involved in human keratinocyte tumor suppression through negative regulation of ROCK1/2 and MRCKa kinases. Genes & Dev. 2007; 21, 562-77
76. Mandinova A, Lefort K, Tommasi di Vignano A, Stonely W, Ostano P, Chiorino G, Iwaki H, Nakanish J, and Dotto GP. The FoxO3a gene is a key negative target of canonical Notch signaling in the keratinocyte UVB-response. EMBO J. 2008, in press.
Reviews:
1. Dotto GP, Horiuchi K, Zinder ND. The origin of DNA replication of bacteriophage fl and its interaction with the phage gene II protein. In: Huberscherm H, Spadari S, eds. Proteins involved in DNA replication. NY: Academic Press, 1984:185-91.
2. Dotto GP, Gilman MZ, Weinberg RA. Multistep carcinogenesis: studies with primary fibroblasts and keratinocytes. In: Langenbach R, Elmore E, Barrett JC, eds. Tumor promoters: biological approaches for mechanistic studies and assay systems. NY: Raven Press, 1987:317-29.
3. Missero C, Dotto GP. p21WAF1/Cip1 and terminal differentiation control of normal epithelia. Molecular and Cellular Differentiation, 1996:4:1-16.
4. Missero C, D'Errico M, Dotto GP, Dogliotti E. * The Molecular Basis of Skin Carcinogenesis. in : The Molecular Basis of Cancer, Acad. Press NY, 1998.
5. Dotto GP. The Keratinocyte Growth/Differentiation Switch. Frontiers in Bioscience, 1998: 3: d502-508.
6. Dotto GP. Signal Transduction Pathways Controlling the Switch between Keratinocyte Growth and Differentiation. Critical Reviews in Oral Biology and Medicine. 1999; 10:
442-457
7. Dotto GP. p21WAF1/Cip1 : more than a break to the cell cycle? Biochemica et
Biophysica Acta, Reviews on Cancer Online, 2000. 8748; 1-14.
8. Lefort K and Dotto GP. Notch signaling in the integrated control of keratinocyte growth/differentiation and tumor suppression. Seminars in Cancer Biology, 2004, 14, 374-386.
9. Dotto GP and Silke J. More than cell death: caspases and caspase inhibitors on the move. Dev Cell. 2004, 7: 2-3.
10. Dotto GP and Cotsarelis G, Rac1 Up for Epidermal Stem Cells. Science, 2005; 309:890-891.
Patents:
Seki, T., Fujii, S. and G.P. Dotto : Use of pyrethroid compounds to promote hair growth, patent # US 6,288,112 B1, Sep 11, 2001

Fujii, S. and G.P. Dotto : Methods and compositions for preventing skin damage, patent # US 7,192,770
 
 
ICFDCR2008 组委会秘书处
地址：北京市朝阳区南朗家园18号恋日国际907室（邮编：100022）